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Congenital disorders of glycosylation and their impact on cellular and energetic metabolism
Rychtárová, Lucie ; Hansíková, Hana (advisor) ; Hodek, Petr (referee) ; Pecinová, Alena (referee)
Inherited disorders of glycosylation (CDG) are a large group of more than 160 types of metabolic disorders caused by genetic defects that lead to impaired glycan biosynthesis and modification. The lipid dolichol plays an essential role in glycan biosynthesis. Glycans play a key role in the function and structure of proteins and lipids and their deficiency leads to severe clinical symptoms. CDG usually manifests in childhood as a multisystem disorder. Families thus face a serious health problem due to the progressive and highly variable nature of the disease, the unfavorable prognosis and, with few exceptions, the unavailability of treatment. Currently, we still do not have a sufficient range of methods to recognize rare types of CDG and our knowledge of the pathophysiology of CDG is still limited. The first aim of this work was to optimize the method of determination of dolichol isoforms and to study them in physiology and pathology. The second aim of the work was to investigate the bioenergetic status and overall metabolism in the most common type of CDG - deficiency of phosphomannomutase 2 (PMM2-CDG), and in CDG caused by a defect in dolichol biosynthesis. The distribution of urinary dolichol isoforms in the population was characterized using an optimized method. The dolichol isoforms profile...
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Soul therapy during the bodywork
Králová, Lada ; Žáčková, Hana (advisor) ; Stretti, Sylvie (referee)
Univerzita Karlova v Praze Husitská teologická fakulta Diplomová práce TERAPIE DUŠE POMOCÍ PRÁCE S TĚLEM BODYTERAPIE Praha, 2012 Bc. Lada Králová Univerzita Karlova v Praze Husitská teologická fakulta Diplomová práce TERAPIE DUŠE POMOCÍ PRÁCE S TĚLEM BODYTERAPIE THERAPY OF THE SOUL BY BODYWORK BODYTHERAPY Vedoucí práce: Autor: PaedDr. Mgr. Hana Žáčková Bc. Lada Králová Praha 2012 Na tomto míst bych ráda pod kovala vedoucí diplomové práce paní PaedDr. Mgr. Haně Žáčkové za cenné připomínky. Prohlášení Prohla uji, e jsem tuto diplomovou práci vypracovala samostatn a výhradn s pou itím citovaných pramen , literatury a dal ích odborných zdroj . v Praze, dne 27. června 2012 Lada Králová Anotace Diplomová práce se zabývá bodyterapií, psychoterapií zaměřenou na tělo. Teoretická část je rozdělena do pěti oddílů, z nichž první část seznamuje s bodyterapií a historií terapeutických přístupů práce s tělem. Druhá ást práce je zaměřena na bioenergetiku a pojetí tělesného konceptu, kterého využívá právě bodyterapie. V další ásti práce se v nujeme rozpoznávání signálů z těla, jako je řeč těla a organismický pohled na osobnost. Ve čtvrté části práce blíže specifikujeme jednotlivé charakterové typy člověka. V posledním oddíle se zabýváme zejména psychoterapeutickou intervencí zaměřenou na tělo. Praktická část mapuje...
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MR spectroscopy in patients with diabetes mellitus
Šedivý, Petr ; Štěpánková, Helena (advisor) ; Roztočil, Karel (referee)
This thesis deals with in vivo MR spectroscopy. Measurements in this thesis were performed on whole-body MR tomograph at the Institute for Clinical and Experimental Medicine in Prague. The objective of the thesis was to study differences in the biochemical processes and energy metabolism in the muscle tissue under physical workload between the groups of healthy subjects and patients with type 1 diabetes mellitus (DM1). We used phosphorous spectroscopy in combination with ergometer. The thesis is divided into five chapters. The first chapter describes theoretical introduction to in vivo 1 H and 31 P MR spectroscopy and muscle metabolism, the second chapter deals with the description of the experimental equipment and measurement, results of the thesis are reported in the third chapter and the fourth chapter is a discussion of results. Main result of this work is summarized in conclusion; we found differences between the metabolism of patients with DM1 and healthy volunteers.
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The role of mitochondrial energy metabolism in cell senescence
Zima, Michal ; Trnka, Jan (advisor) ; Pecinová, Alena (referee)
Cellular senescence represents status, when the cells cease to divide and remain in permanent cell cycle arrest. Senescence is considered to be an active response of the cell to various extrinsic and intrinsic types of stress such as certain oncogene activation, exposing to several cytokines or drugs and damaged and/or uncapped telomeres. Senescent cells are characterised by extensive modification of gene expression, flattened and enlargement of cellular body. Hypothetically, altered gene expression may lead also to increase of certain surface proteins expression. Such protein can be L1 cell adhesive molecule (L1CAM), which is expressed heterogeneously within the population. This Thesis describes current knowledge of cellular senescence, the mechanism, which may result in establishment of senescence phenotype, and also the characteristic markers of senescence. Thesis also puts together the heterogeneity of L1CAM expression in A375 senescent cells with oxygen consumption rate and extracellular acidification rate performed by Seahorse XFe24 metabolic analyser. Therefore, ells were sorted according to their levels of expressing L1CAM onto low and high L1CAM expressing subpopulations. Obtained data show potential correlation between the rate of L1CAM expression in A375 cells and the metabolic rate. Key...
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Studium technologií zpracování biodegradabilních odpadů z trvalých porostů pro energetické účely
Michálek, Milan
The dissertation thesis entitled "Study of technologies for processing biodegradable waste from permanent crops for energy purposes" is about the use of waste wood material generated after the pruning of crops (vineyards and orchards). The processing of waste biomass from these crops can be used machinery, such as shredders, shredders with reservoir and shredders. In this theses were carried out a series of monitoring of times and computing performance of different machine sets in different conditions. Individual monitoring varied by the use of machinery, cultivation of the variety series length, slope of terrain, spacing, etc. The recalculated performance then ranges from 0,41 to 0,77 hectares per hour. From the time measurements and calculations of costs were for each variant of processing of the vine cane determined values of costs from 880 to 1,357 CZK per ton of wood chips and from 941 to 1,184 CZK per tonne of vine cane compressed into the bales. For the processing of waste wood from orchards, the resulting values were 909--1742 per ton of wood chips and 1128--1505 per tonne of bales. As the most economically advantageous technological progress of processing the vince cane seems to be shredding to the trailer attached behind the shredder. For this method, the resulting cost of wood chips range from 880 CZK per tonne. The same technological process is also proven in the orchards, where the resulting costs per ton of wood chips is 909 CZK. These results confirm that testing technologies provide a competitive bioenergy products. Economic evaluation of monitored technologies depending on the deployment shows that these technologies can be implemented effectively in enterprises with horticultural areas over 45 ha. This area corresponds to medium and large wine-growing and fruit growing enterprise
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The role of mitochondrial energy metabolism in cell senescence
Zima, Michal ; Trnka, Jan (advisor) ; Pecinová, Alena (referee)
Cellular senescence represents status, when the cells cease to divide and remain in permanent cell cycle arrest. Senescence is considered to be an active response of the cell to various extrinsic and intrinsic types of stress such as certain oncogene activation, exposing to several cytokines or drugs and damaged and/or uncapped telomeres. Senescent cells are characterised by extensive modification of gene expression, flattened and enlargement of cellular body. Hypothetically, altered gene expression may lead also to increase of certain surface proteins expression. Such protein can be L1 cell adhesive molecule (L1CAM), which is expressed heterogeneously within the population. This Thesis describes current knowledge of cellular senescence, the mechanism, which may result in establishment of senescence phenotype, and also the characteristic markers of senescence. Thesis also puts together the heterogeneity of L1CAM expression in A375 senescent cells with oxygen consumption rate and extracellular acidification rate performed by Seahorse XFe24 metabolic analyser. Therefore, ells were sorted according to their levels of expressing L1CAM onto low and high L1CAM expressing subpopulations. Obtained data show potential correlation between the rate of L1CAM expression in A375 cells and the metabolic rate. Key...
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MR spectroscopy in patients with diabetes mellitus
Šedivý, Petr ; Štěpánková, Helena (advisor) ; Roztočil, Karel (referee)
This thesis deals with in vivo MR spectroscopy. Measurements in this thesis were performed on whole-body MR tomograph at the Institute for Clinical and Experimental Medicine in Prague. The objective of the thesis was to study differences in the biochemical processes and energy metabolism in the muscle tissue under physical workload between the groups of healthy subjects and patients with type 1 diabetes mellitus (DM1). We used phosphorous spectroscopy in combination with ergometer. The thesis is divided into five chapters. The first chapter describes theoretical introduction to in vivo 1 H and 31 P MR spectroscopy and muscle metabolism, the second chapter deals with the description of the experimental equipment and measurement, results of the thesis are reported in the third chapter and the fourth chapter is a discussion of results. Main result of this work is summarized in conclusion; we found differences between the metabolism of patients with DM1 and healthy volunteers.
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The legacy of Wilhelm Reich and its reflexion in the work of the founders of Bioenergetics, Biosynthesis and Biodynamic Psychology
Vaněčková, Marta ; Pivoňková, Věra (advisor) ; Hroníková, Linda (referee)
The diploma thesis The legacy of Wilhelm Reich and its reflexion in the work of the founders of Bioenergetics, Biosynthesis and Biodynamic psychology is devoted to a comparison of the most important theoretical concepts and therapeutic techniques of Wilhelm Reich with the methods of the founders of selected psychotherapeutic approaches that draw from his work. These are the founder of Bioenergetics Alexander Lowen, founder of the Biosynthesis David Boadella and founder of Biodynamic Psychology Gerda Boyesen. These authors created distinct therapeutic approaches based on Reich's concepts that significantly shaped the field of the Body- Psychotherapy. The first part explains the technique of Character-Analysis and Vegetotherapy, the Orgasm Theory (the role of sexuality in the etiology of neurosis), a typology of Character structures, the Orgonomic Research and principles of the Orgonomic Functionalism, which Reich defined and used in practice. There is briefly introduced the history of the development within the Neoreichian movement (Reich pupils and followers) in the second half of the 20th century. The vast majority of the second part of the thesis is devoted to reflection on Reich's work in the selected body-psychotherapeutic schools and the way they work with his legacy. The Bioenergetics is...
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Effect of ski-mountaineering equipment on energy expenditure of skiing
Smětáková, Martina ; Baláš, Jiří (advisor) ; Jindra, Matouš (referee)
Title of master thesis Effect of ski-mountaineering equipment on energy expenditure of skiing. Work objectives The aim of this study was to determine the effect of weight ski equipment energy performance when walking on snow. Methods The study included 6 skialpinists a mean age of 30 ± 5.5 years. Energy intensity was measured on the basis of breathe oxygen (VO2) and exhaled (VCO2). The measurements were used 3 types of ski-hiking equipment (light - medium x 3160 g - 7754 g x heavy - 9600 g). Each test first completed section in a time of 6 minutes flat terrain (0ř), after which he immediately set out to climb the 360 m long route at an angle (20ř). These two sections (0ř and 20ř) graduated in random order for each type of equipment and at a constant speed of 1 m·s-1 . Results The results show that energy intensity decreases significantly when using ultralight ski equipment. We found that the climb at an angle of 20ř is the difference in oxygen consumption (VO2) between moderate and light equipment 9.6% between heavy and light equipment 17.7% and medium-heavy and heavy equipment 7,4%. The average VO2 test with light equipment was 44.4 ± 3.7 ml·kg-1 ·min-1 , with moderate equipment 48.7 ± 5.6 ml·kg- 1 ·min-1 and heavy equipment 52.2 ± 4.8 ml·kg-1 ·min-1 . At 0ř inclination and moderate use of heavy...
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Soul therapy during the bodywork
Králová, Lada ; Žáčková, Hana (advisor) ; Stretti, Sylvie (referee)
Univerzita Karlova v Praze Husitská teologická fakulta Diplomová práce TERAPIE DUŠE POMOCÍ PRÁCE S TĚLEM BODYTERAPIE Praha, 2012 Bc. Lada Králová Univerzita Karlova v Praze Husitská teologická fakulta Diplomová práce TERAPIE DUŠE POMOCÍ PRÁCE S TĚLEM BODYTERAPIE THERAPY OF THE SOUL BY BODYWORK BODYTHERAPY Vedoucí práce: Autor: PaedDr. Mgr. Hana Žáčková Bc. Lada Králová Praha 2012 Na tomto míst bych ráda pod kovala vedoucí diplomové práce paní PaedDr. Mgr. Haně Žáčkové za cenné připomínky. Prohlášení Prohla uji, e jsem tuto diplomovou práci vypracovala samostatn a výhradn s pou itím citovaných pramen , literatury a dal ích odborných zdroj . v Praze, dne 27. června 2012 Lada Králová Anotace Diplomová práce se zabývá bodyterapií, psychoterapií zaměřenou na tělo. Teoretická část je rozdělena do pěti oddílů, z nichž první část seznamuje s bodyterapií a historií terapeutických přístupů práce s tělem. Druhá ást práce je zaměřena na bioenergetiku a pojetí tělesného konceptu, kterého využívá právě bodyterapie. V další ásti práce se v nujeme rozpoznávání signálů z těla, jako je řeč těla a organismický pohled na osobnost. Ve čtvrté části práce blíže specifikujeme jednotlivé charakterové typy člověka. V posledním oddíle se zabýváme zejména psychoterapeutickou intervencí zaměřenou na tělo. Praktická část mapuje...
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